7U4D

CryoEM structure of CENP-N promoted nucleosome stacks with CENP-A and 601 DNA sequence

Exp. Method EM
Resolution, Å 8.1
Release Date 2022-03-30
Update Date 2024-02-21
Authors Zhou, K., Gebala, M., Woods, D., Sundararajan, K., Edwards, G., Krzizike, D., Wereszczynski, J., Straight, A.F., Luger, K.
Publication PMID: 35422519
doi 10.1038/s41594-022-00758-y
Related structures 6C0W
RCSB PDB 7U4D
EMDB EMD-26332
Q-score 0.114
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal A cenH3 Homo sapiens P49450
Distal E cenH3 Homo sapiens P49450
H4 Proximal B canonical H4 Homo sapiens P62805
Distal F canonical H4 Homo sapiens P62805
H2A Proximal C canonical H2A Homo sapiens Q93077
Distal G canonical H2A Homo sapiens Q93077
H2B Proximal D canonical H2B Homo sapiens P62807
Distal H canonical H2B Homo sapiens P62807
Other proteins:
Chain Name UID
K Centromere protein N Q96H22
V Centromere protein N Q96H22
DNA:
Chain Variant Length
I 601 139
J 601 139
T 601 139
U 601 139

H3

H4

H2A

H2B

DNA

Other


Chain Bfactor Q-score Local Resolution

Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.