8SN7

Cryo-EM structure of the human nucleosome core particle in complex with RNF168 and UbcH5c~Ub (UbcH5c chemically conjugated to histone H2A) (class 5)

Exp. Method EM
Resolution, Å 3.7
Release Date 2024-01-17
Update Date 2024-03-20
Authors Hu, Q., Zhao, D., Cui, G., Bhandari, J., Thompson, J.R., Botuyan, M.V., Mer, G.
Publication PMID: 38242129
doi 10.1016/j.molcel.2023.12.036
RCSB PDB 8SN7
EMDB EMD-40615
Q-score 0.445
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal A canonical H3 Homo sapiens P68431
Distal E canonical H3 Homo sapiens P68431
H4 Proximal B canonical H4 Homo sapiens P62805
Distal F canonical H4 Homo sapiens P62805
H2A Proximal C canonical H2A Homo sapiens P04908
Distal G canonical H2A Homo sapiens P04908
H2B Proximal D canonical H2B Homo sapiens P06899
Distal H canonical H2B Homo sapiens P06899
Other proteins:
Chain Name UID
K E3 ubiquitin-protein ligase RNF168 Q8IYW5
L Ubiquitin-conjugating enzyme E2 D3 P61077
M Polyubiquitin-B P0CG47
DNA:
Chain Variant Length
I 601 147
J 601 147

H3

H4

H2A

H2B

DNA

Other


Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.