8OW0

Cryo-EM structure of CBF1-CCAN bound topologically to a centromeric CENP-A nucleosome

Exp. Method EM
Resolution, Å 3.4
Release Date 2023-08-09
Update Date 2023-08-09
Authors Dendooven, T., Zhang, Z., Yang, J., McLaughlin, S.H., Schwab, J., Scheres, S.H.W., Yatskevich, S., Barford, D.
Publication PMID: 37506202
doi 10.1126/sciadv.adg7480
RCSB PDB 8OW0
EMDB EMD-17226
Q-score 0.2
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal a cenH3 Saccharomyces cerevisiae P36012
Distal e cenH3 Saccharomyces cerevisiae P36012
H4 Proximal b canonical H4 Saccharomyces cerevisiae P02309
Distal f canonical H4 Saccharomyces cerevisiae P02309
H2A Proximal c H2A.X Saccharomyces cerevisiae P04911
Distal g H2A.X Saccharomyces cerevisiae P04911
H2B Proximal d canonical H2B Saccharomyces cerevisiae P02293
Distal h canonical H2B Saccharomyces cerevisiae P02293
Other proteins:
Chain Name UID
A Centromere-binding protein 1 P17106
B Centromere-binding protein 1 P17106
H Inner kinetochore subunit MCM16 Q12262
I Inner kinetochore subunit CTF3 Q12748
K Inner kinetochore subunit MCM22 P47167
L Inner kinetochore subunit IML3 P38265
N Inner kinetochore subunit CHL4 P38907
O Inner kinetochore subunit MCM21 Q06675
P Inner kinetochore subunit CTF19 Q02732
Q Inner kinetochore subunit OKP1 P53298
T Inner kinetochore subunit CNN1 P43618
U Inner kinetochore subunit AME1 P38313
W Inner kinetochore subunit WIP1 Q2V2P8
Y Inner kinetochore subunit NKP1 Q12493
Z Inner kinetochore subunit NKP2 Q06162
DNA:
Chain Variant Length
D 601_modified 121
E 601_modified 120

H3

H4

H2A

H2B

DNA

Other


Chain Bfactor Q-score

Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.