8JNE

The cryo-EM structure of the decameric RAD51 ring bound to the nucleosome without the linker DNA binding

Exp. Method EM
Resolution, Å 4.68
Release Date 2024-03-27
Update Date 2024-05-08
Authors Shioi, T., Hatazawa, S., Oya, E., Hosoya, N., Kobayashi, W., Ogasawara, M., Kobayashi, T., Takizawa, Y., Kurumizaka, H.
Publication PMID: 38509361
doi 10.1038/s41586-024-07196-4
RCSB PDB 8JNE
EMDB EMD-36443
Q-score 0.13
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal E canonical H3 Homo sapiens P68431
Distal A canonical H3 Homo sapiens P68431
H4 Proximal F canonical H4 Homo sapiens P62805
Distal B canonical H4 Homo sapiens P62805
H2A Proximal G canonical H2A Homo sapiens P04908
Distal C canonical H2A Homo sapiens P04908
H2B Proximal H canonical H2B Homo sapiens P06899
Distal D canonical H2B Homo sapiens P06899
Other proteins:
Chain Name UID
K DNA repair protein RAD51 homolog 1 Q06609
L DNA repair protein RAD51 homolog 1 Q06609
M DNA repair protein RAD51 homolog 1 Q06609
N DNA repair protein RAD51 homolog 1 Q06609
O DNA repair protein RAD51 homolog 1 Q06609
P DNA repair protein RAD51 homolog 1 Q06609
Q DNA repair protein RAD51 homolog 1 Q06609
R DNA repair protein RAD51 homolog 1 Q06609
S DNA repair protein RAD51 homolog 1 Q06609
T DNA repair protein RAD51 homolog 1 Q06609
DNA:
Chain Variant Length
I 601_modified 156
J 601_modified 153

H3

H4

H2A

H2B

DNA

Other


Chain Bfactor Q-score

Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.