8H0W

RNA polymerase II transcribing a chromatosome (type II)

Exp. Method EM
Resolution, Å 4.6
Release Date 2022-12-07
Update Date 2022-12-07
Authors Hirano, R., Ehara, H., Kujirai, T., Uejima, T., Takizawa, Y., Sekine, S.I., Kurumizaka, H.
Publication PMID: 36435862
doi 10.1038/s41467-022-35003-z
RCSB PDB 8H0W
EMDB EMD-34416
Q-score 0.16
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal a canonical H3 Homo sapiens P68431
Distal e canonical H3 Homo sapiens P68431
H4 Proximal b canonical H4 Homo sapiens P62805
Distal f canonical H4 Homo sapiens P62805
H2A Proximal c canonical H2A Homo sapiens P04908
Distal g canonical H2A Homo sapiens P04908
H2B Proximal d canonical H2B Homo sapiens P06899
Distal h canonical H2B Homo sapiens P06899
H1:
Chain HistoneDB Variant UID
u H1.2 P16403
Other proteins:
Chain Name UID
A DNA-directed RNA polymerase subunit C4R4Y0
B DNA-directed RNA polymerase subunit beta C4QZQ7
C RNA polymerase II third largest subunit B44, part of central core C4R7L2
D RNA polymerase II subunit B32 C4R2U9
E DNA-directed RNA polymerases I, II, and III subunit RPABC1 C4R3P8
F RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III C4R1V1
G RNA polymerase II subunit C4R9A1
H DNA-directed RNA polymerases I, II, and III subunit RPABC3 C4R273
I DNA-directed RNA polymerase subunit F2QPE6
J RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III C4R009
K RNA polymerase II subunit B12.5 C4R3Z5
L RNA polymerase subunit ABC10-alpha F2QMI1
DNA:
Chain Variant Length
N 601 196
T 601 205

H3

H4

H2A

H2B

H1

DNA

Other


Chain Bfactor Q-score Local Resolution

Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.