8BYQ

RNA polymerase II pre-initiation complex with the proximal +1 nucleosome (PIC-Nuc10W)

Exp. Method EM
Resolution, Å 4.1
Release Date 2023-05-03
Update Date 2023-06-14
Authors Abril-Garrido, J., Dienemann, C., Grabbe, F., Velychko, T., Lidschreiber, M., Wang, H., Cramer, P.
Publication PMID: 37148879
doi 10.1016/j.molcel.2023.04.011
Related structures 8BVW, 8BZ1
RCSB PDB 8BYQ
EMDB EMD-16331
Q-score 0.187
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal a canonical H3 Xenopus laevis P84233
Distal e canonical H3 Xenopus laevis P84233
H4 Proximal b canonical H4 Xenopus laevis P62799
Distal f canonical H4 Xenopus laevis P62799
H2A Proximal c canonical H2A Xenopus laevis P06897
Distal g canonical H2A Xenopus laevis P06897
H2B Proximal d canonical H2B Xenopus laevis P02281
Distal h canonical H2B Xenopus laevis P02281
Other proteins:
Chain Name UID
0 General transcription and DNA repair factor IIH helicase subunit XPB P19447
1 TFIIH basal transcription factor complex helicase XPD subunit P18074
2 General transcription factor IIH subunit 1 P32780
3 General transcription factor IIH subunit 4 Q92759
4 General transcription factor IIH subunit 2 Q13888
5 General transcription factor IIH subunit 3 Q13889
6 General transcription factor IIH subunit 5 Q6ZYL4
7 CDK-activating kinase assembly factor MAT1 P51948
8 Cyclin-dependent kinase 7 P50613
9 Cyclin-H P51946
A DNA-directed RNA polymerase subunit A0A7M4DUC2
B DNA-directed RNA polymerase subunit beta I3LGP4
C DNA-directed RNA polymerase II subunit RPB3 I3LCH3
D RNA polymerase II subunit D A0A287ADR4
E DNA-directed RNA polymerase II subunit E I3LSI7
F DNA-directed RNA polymerase II subunit F A0A4X1VEK9
G DNA-directed RNA polymerase II subunit RPB7 A0A4X1VKG7
H DNA-directed RNA polymerases I, II, and III subunit RPABC3 I3LCB2
I DNA-directed RNA polymerase II subunit RPB9 P60899
J DNA-directed RNA polymerases I, II, and III subunit RPABC5 A0A4X1VYD0
K DNA-directed RNA polymerase II subunit RPB11-a F1RKE4
L RNA polymerase II subunit K A0A4X1TRS6
M Transcription initiation factor IIB Q00403
O TATA-box-binding protein P20226
Q General transcription factor IIF subunit 1 P35269
R General transcription factor IIF subunit 2 P13984
U Transcription initiation factor IIA subunit 1 P52655
V Transcription initiation factor IIA subunit 2 P52657
W General transcription factor IIE subunit 1 P29083
X Transcription initiation factor IIE subunit beta P29084
DNA:
Chain Variant Length
N 601 198
T 601 198

H3

H4

H2A

H2B

DNA

Other


Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.