8A3Y

Structure of mammalian Pol II-DSIF-SPT6-PAF1-TFIIS-hexasome elongation complex

Exp. Method EM
Resolution, Å 3.3
Release Date 2024-07-31
Update Date 2024-11-06
Authors Farnung, L., Ochmann, M., Garg, G., Vos, S.M., Cramer, P.
doi 10.1016/j.molcel.2022.06.027
Publication PMID: 35858621
Related structures
RCSB PDB 8A3Y
EMDB EMD-15127
Q-score 0.231
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal a canonical H3 Xenopus laevis P84233
Distal e canonical H3 Xenopus laevis P84233
H4 Proximal b canonical H4 Xenopus laevis P62799
Distal f canonical H4 Xenopus laevis P62799
H2A Proximal c canonical H2A Xenopus laevis Q6AZJ8
H2B Proximal d canonical H2B Xenopus laevis A0A8J1LZU9
Other proteins:
Chain Name UID
A DNA-directed RNA polymerase subunit A0A7M4DUC2
B DNA-directed RNA polymerase subunit beta I3LGP4
C DNA-directed RNA polymerase II subunit RPB3 I3LCH3
D RNA polymerase II subunit D A0A287ADR4
E DNA-directed RNA polymerase II subunit E I3LSI7
F DNA-directed RNA polymerases I, II, and III subunit RPABC2 A0A4X1VEK9
G DNA-directed RNA polymerase II subunit RPB7 A0A4X1VKG7
H DNA-directed RNA polymerases I, II, and III subunit RPABC3 I3LCB2
I DNA-directed RNA polymerase II subunit RPB9 P60899
J DNA-directed RNA polymerases I, II, and III subunit RPABC5 A0A4X1VYD0
K DNA-directed RNA polymerase II subunit RPB11-a F1RKE4
L RNA polymerase II subunit K A0A8D0JYF1
M SPT6 Q7KZ85
Q RNA polymerase-associated protein CTR9 homolog,RNA polymerase-associated protein LEO1 Q6PD62
R RNA polymerase-associated protein RTF1 homolog Q92541
U RNA polymerase-associated protein CTR9 homolog,RNA polymerase-associated protein LEO1 Q6PD62
V RNA polymerase II-associated factor 1 homolog Q8N7H5
W WD repeat-containing protein 61 Q9GZS3
X Parafibromin Q6P1J9
Y Transcription elongation factor SPT4 P63272
Z Transcription elongation factor SPT5 O00267
DNA:
Chain Variant Length
N 601_modified 127
T 601_modified 138

H3

H4

H2A

H2B

DNA

Other


Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.