7Y5W

Cryo-EM structure of the left-handed Di-tetrasome

Exp. Method	EM
Resolution, Å	3.5
Release Date	2023-08-16
Update Date	2023-09-06
Authors	Liu, C.P., Yu, Z., Xiong, J., Hu, J., Song, A., Ding, D., Yu, C., Yang, N., Wang, M., Yu, J., Hou, P., Zeng, K., Li, Z., Zhang, Z., Zhang, X., Li, W., Zhang, Z., Zhu, B., Li, G., Xu, R.M.
Publication	PMID: 37616371
doi	10.1126/science.add8673
RCSB PDB	7Y5W
EMDB	EMD-33627
Q-score	0.35

Core histones in Nucleosome-like particles:

	Side	Chain	HistoneDB Variant	Taxa	UID
H3	Proximal	C	canonical H3	Homo sapiens	P68431
H3	Distal	A	canonical H3	Homo sapiens	P68431
H4	Proximal	D	canonical H4	Homo sapiens	P62805
H4	Distal	B	canonical H4	Homo sapiens	P62805

	Side	Chain	HistoneDB Variant	Taxa	UID
H3	Distal	E	canonical H3	Homo sapiens	P68431
H3	Distal	G	canonical H3	Homo sapiens	P68431
H4	Distal	F	canonical H4	Homo sapiens	P62805
H4	Distal	H	canonical H4	Homo sapiens	P62805

DNA:

Chain	Variant	Length
I	601	101
J	601	101

H3

H4

H2A

H2B

DNA

Other

Orthographic Superimpose 1KX5 Show axes

Aligned nucleosome coordinates

X - Y

X - Z

Z - Y

Analysis fail

Nucleosome reference frame definition

DNA relative twist and local base step parameters

3DNA

Relative Twist

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.

H3d chain A

H4d chain B

H3p chain C

H4p chain D

DNA top chain I, bot chain J

Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.