7WLR

Cryo-EM structure of the nucleosome containing Komagataella pastoris histones

Exp. Method EM
Resolution, Å 3.54
Release Date 2022-07-13
Update Date 2022-08-10
Authors Fukushima, Y., Hatazawa, S., Hirai, S., Kujirai, T., Ehara, H., Sekine, S.I., Takizawa, Y., Kurumizaka, H.
Publication PMID: 35485963
doi 10.1093/jb/mvac043
RCSB PDB 7WLR
EMDB EMD-32591
Q-score 0.45
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal A H3.3 Komagataella pastoris A0A1B2JB78
Distal E H3.3 Komagataella pastoris A0A1B2JB78
H4 Proximal B canonical H4 Komagataella pastoris A0A1B2JA70
Distal F canonical H4 Komagataella pastoris A0A1B2JA70
H2A Proximal C H2A.X Komagataella pastoris A0A1B2JD99
Distal G H2A.X Komagataella pastoris A0A1B2JD99
H2B Proximal D canonical H2B Komagataella pastoris A0A1B2JBS1
Distal H canonical H2B Komagataella pastoris A0A1B2JBS1
DNA:
Chain Variant Length
I 601 145
J 601 145

H3

H4

H2A

H2B

DNA

Other


Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.