7UNC

Pol II-DSIF-SPT6-PAF1c-TFIIS complex with rewrapped nucleosome

Exp. Method EM
Resolution, Å 3
Release Date 2022-10-19
Update Date 2022-10-19
Authors Filipovski, M., Soffers, J.H.M., Vos, S.M., Farnung, L.
Publication PMID: 35709268
doi 10.1126/science.abo3851
RCSB PDB 7UNC
EMDB EMD-26620
Q-score 0.344
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal a canonical H3 Xenopus laevis P84233
Distal e canonical H3 Xenopus laevis P84233
H4 Proximal b canonical H4 Xenopus laevis P62799
Distal f canonical H4 Xenopus laevis P62799
H2A Proximal c canonical H2A Xenopus laevis Q6AZJ8
Distal g canonical H2A Xenopus laevis Q6AZJ8
H2B Proximal d canonical H2B Xenopus laevis P02281
Distal h canonical H2B Xenopus laevis P02281
Other proteins:
Chain Name UID
A DNA-directed RNA polymerase subunit A0A7M4DUC2
B DNA-directed RNA polymerase subunit beta A0A4X1TVZ5
C DNA-directed RNA polymerase II subunit RPB3 A0A481DF93
D RPOL4c domain-containing protein A0A8D1GCS3
E DNA-directed RNA polymerase II subunit E A0A4X1VTX4
F DNA-directed RNA polymerases I, II, and III subunit RPABC2 A0A4X1VEK9
G DNA-directed RNA polymerase II subunit RPB7 A0A4X1VKG7
H RPB8 NA
I RPB9 NA
J RPB10 NA
K RPB11 NA
L RNA polymerase II subunit K A0A4X1TRS6
M Transcription elongation factor SPT6 Q7KZ85
O Transcription elongation factor A protein 1 P23193
Q RNA polymerase-associated protein CTR9 homolog Q6PD62
R RNA polymerase-associated protein RTF1 homolog Q92541
U RNA polymerase-associated protein LEO1 Q8WVC0
V RNA polymerase II-associated factor 1 homolog Q8N7H5
W WDR61 Q9GZS3
X Parafibromin Q6P1J9
Z Transcription elongation factor SPT5 O00267
DNA:
Chain Variant Length
N 601 140
T 601_modified 155

H3

H4

H2A

H2B

DNA

Other


Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.