7SSA

Cryo-EM structure of pioneer factor Cbf1 bound to the nucleosome

Exp. Method EM
Resolution, Å 3.2
Release Date 2023-04-05
Update Date 2023-05-03
Authors Donovan, B.T., Chen, H., Eek, P., Meng, Z., Jipa, C., Tan, S., Bai, L., Poirier, M.G.
Publication PMID: 36996811
doi 10.1016/j.molcel.2023.03.006
RCSB PDB 7SSA
EMDB EMD-25406
Q-score 0.449
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal A canonical H3 Xenopus laevis P84233
Distal E canonical H3 Xenopus laevis P84233
H4 Proximal B canonical H4 Xenopus laevis P62799
Distal F canonical H4 Xenopus laevis P62799
H2A Proximal C H2A.X Saccharomyces cerevisiae (strain ATCC 204508 / S288c) P04911
Distal G H2A.X Saccharomyces cerevisiae (strain ATCC 204508 / S288c) P04911
H2B Proximal D canonical H2B Saccharomyces cerevisiae (strain ATCC 204508 / S288c) P02293
Distal H canonical H2B Saccharomyces cerevisiae (strain ATCC 204508 / S288c) P02293
Other proteins:
Chain Name UID
K Centromere-binding protein 1 P17106
L Centromere-binding protein 1 P17106
DNA:
Chain Variant Length
I 601 137
J 601 137

H3

H4

H2A

H2B

DNA

Other


Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.