7LYB

Cryo-EM structure of the human nucleosome core particle in complex with BRCA1-BARD1-UbcH5c

Exp. Method EM
Resolution, Å 3.28
Release Date 2021-07-28
Update Date 2021-09-01
Authors Hu, Q., Botuyan, M.V., Zhao, D., Cui, G., Mer, E., Mer, G.
Publication PMID: 34321665
doi 10.1038/s41586-021-03716-8
RCSB PDB 7LYB
EMDB EMD-23591
Q-score 0.458
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal A canonical H3 Homo sapiens P68431
Distal E canonical H3 Homo sapiens P68431
H4 Proximal B canonical H4 Homo sapiens P62805
Distal F canonical H4 Homo sapiens P62805
H2A Proximal C canonical H2A Homo sapiens P04908
Distal G canonical H2A Homo sapiens P04908
H2B Proximal D canonical H2B Homo sapiens P06899
Distal H canonical H2B Homo sapiens P06899
Other proteins:
Chain Name UID
M Isoform 7 of Breast cancer type 1 susceptibility protein P38398
N BRCA1-associated RING domain protein 1 Q99728
P Ubiquitin-conjugating enzyme E2 D3 P61077
DNA:
Chain Variant Length
I 601 147
J 601 146

H3

H4

H2A

H2B

DNA

Other


Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.