7CCR

Structure of the 2:2 cGAS-nucleosome complex

Exp. Method	EM
Resolution, Å	4.9
Release Date	2020-10-07
Update Date	2024-03-27
Authors	Cao, D., Han, X., Fan, X., Xu, R.M., Zhang, X.
Publication	PMID: 33051594
doi	10.1038/s41422-020-00422-4
RCSB PDB	7CCR
EMDB	EMD-30340
Q-score	0.152

Core histones in Nucleosome-like particles:

	Side	Chain	HistoneDB Variant	Taxa	UID
H3	Proximal	A	canonical H3	Homo sapiens	P68431
H3	Distal	E	canonical H3	Homo sapiens	P68431
H4	Proximal	B	canonical H4	Homo sapiens	P62805
H4	Distal	F	canonical H4	Homo sapiens	P62805
H2A	Proximal	C	canonical H2A	Homo sapiens	P04908
H2A	Distal	G	canonical H2A	Homo sapiens	P04908
H2B	Proximal	D	canonical H2B	Homo sapiens	P06899
H2B	Distal	H	canonical H2B	Homo sapiens	P06899

	Side	Chain	HistoneDB Variant	Taxa	UID
H3	Proximal	L	canonical H3	Homo sapiens	P68431
H3	Proximal	P	canonical H3	Homo sapiens	P68431
H4	Proximal	M	canonical H4	Homo sapiens	P62805
H4	Proximal	Q	canonical H4	Homo sapiens	P62805
H2A	Proximal	N	canonical H2A	Homo sapiens	P04908
H2A	Proximal	R	canonical H2A	Homo sapiens	P04908
H2B	Proximal	O	canonical H2B	Homo sapiens	P06899
H2B	Proximal	S	canonical H2B	Homo sapiens	P06899

Other proteins:

Chain	Name	UID
K	Cyclic GMP-AMP synthase	Q8N884
V	Cyclic GMP-AMP synthase	Q8N884

DNA:

Chain	Variant	Length
I	601	147
J	601	147
T	601	147
U	601	147

H3

H4

H2A

H2B

DNA

Other

Orthographic Superimpose 1KX5 Show axes

Aligned nucleosome coordinates

X - Y

X - Z

Z - Y

Nucleosome reference frame definition

DNA relative twist and local base step parameters

3DNA

Relative Twist

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.

H3p chain A

H4p chain B

H2Ap chain C

H2Bp chain D

H3d chain E

H4d chain F

H2Ad chain G

H2Bd chain H

DNA top chain I, bot chain J

Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.