7C0M

Human cGAS-nucleosome complex

Exp. Method	EM
Resolution, Å	3.9
Release Date	2020-09-16
Update Date	2024-03-27
Authors	Kujirai, T., Zierhut, C., Takizawa, Y., Kim, R., Negishi, L., Uruma, N., Hirai, S., Funabiki, H., Kurumizaka, H.
Publication	PMID: 32912999
doi	10.1126/science.abd0237
RCSB PDB	7C0M
EMDB	EMD-30267
Q-score	0.225

Core histones in Nucleosome-like particles:

	Side	Chain	HistoneDB Variant	Taxa	UID
H3	Proximal	A	canonical H3	Homo sapiens	P68431
H3	Distal	E	canonical H3	Homo sapiens	P68431
H4	Proximal	B	canonical H4	Homo sapiens	P62805
H4	Distal	F	canonical H4	Homo sapiens	P62805
H2A	Proximal	C	canonical H2A	Homo sapiens	P04908
H2A	Distal	G	canonical H2A	Homo sapiens	P04908
H2B	Proximal	D	canonical H2B	Homo sapiens	P06899
H2B	Distal	H	canonical H2B	Homo sapiens	P06899

	Side	Chain	HistoneDB Variant	Taxa	UID
H3	Proximal	e	canonical H3	Homo sapiens	P68431
H3	Distal	a	canonical H3	Homo sapiens	P68431
H4	Proximal	f	canonical H4	Homo sapiens	P62805
H4	Distal	b	canonical H4	Homo sapiens	P62805
H2A	Proximal	g	canonical H2A	Homo sapiens	P04908
H2A	Distal	c	canonical H2A	Homo sapiens	P04908
H2B	Proximal	h	canonical H2B	Homo sapiens	P06899
H2B	Distal	d	canonical H2B	Homo sapiens	P06899

Other proteins:

Chain	Name	UID
K	Cyclic GMP-AMP synthase	Q8N884
k	Cyclic GMP-AMP synthase	Q8N884

DNA:

Chain	Variant	Length
I	601	145
J	601	145
i	601	145
j	601	145

H3

H4

H2A

H2B

DNA

Other

Orthographic Superimpose 1KX5 Show axes

Aligned nucleosome coordinates

X - Y

X - Z

Z - Y

Nucleosome reference frame definition

DNA relative twist and local base step parameters

3DNA

Relative Twist

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.

H3p chain A

H4p chain B

H2Ap chain C

H2Bp chain D

H3d chain E

H4d chain F

H2Ad chain G

H2Bd chain H

DNA top chain I, bot chain J

Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.