7BXT

The cryo-EM structure of CENP-A nucleosome in complex with CENP-C peptide and CENP-N N-terminal domain

Exp. Method EM
Resolution, Å 4.2
Release Date 2021-02-10
Update Date 2024-03-27
Authors Ariyoshi, M., Makino, F., Watanabe, R., Nakagawa, R., Kato, T., Namba, K., Arimura, Y., Fujita, R., Kurumizaka, H., Okumura, E.I., Hara, M., Fukagawa, T.
Publication PMID: 33463726
doi 10.15252/embj.2020105671
RCSB PDB 7BXT
EMDB EMD-30237
Q-score 0.387
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal A canonical H3 Gallus gallus P84229
Distal E canonical H3 Gallus gallus P84229
H4 Proximal B canonical H4 Homo sapiens P62805
Distal F canonical H4 Homo sapiens P62805
H2A Proximal C canonical H2A Homo sapiens P04908
Distal G canonical H2A Homo sapiens P04908
H2B Proximal D canonical H2B Homo sapiens Q16778
Distal H canonical H2B Homo sapiens Q16778
Other proteins:
Chain Name UID
K CENP-C O57392
L CENP-C O57392
M Maltodextrin-binding protein,Centromere protein N P0AEX9
N Maltodextrin-binding protein,Centromere protein N P0AEX9
DNA:
Chain Variant Length
I 601 145
J 601 145

H3

H4

H2A

H2B

DNA

Other


Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.