6J99

Cryo-EM structure of human DOT1L in complex with an H2B-monoubiquitinated nucleosome

Exp. Method EM
Resolution, Å 4.1
Release Date 2019-02-27
Update Date 2019-04-10
Authors Yao, T., Jing, W., Hu, Z., Tan, M., Cao, M., Wang, Q., Li, Y., Yuan, G., Lei, M., Huang, J.
Publication PMID: 30770869
doi 10.1038/s41422-019-0146-7
RCSB PDB 6J99
EMDB EMD-9783
Q-score 0.132
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal A canonical H3 Xenopus laevis A0A310TTQ1
Distal E canonical H3 Xenopus laevis A0A310TTQ1
H4 Proximal B canonical H4 Xenopus laevis P62799
Distal F canonical H4 Xenopus laevis P62799
H2A Proximal C canonical H2A Xenopus laevis Q6AZJ8
Distal G canonical H2A Xenopus laevis Q6AZJ8
H2B Proximal D canonical H2B Xenopus laevis P02281
Distal H canonical H2B Xenopus laevis P02281
Other proteins:
Chain Name UID
K Histone-lysine N-methyltransferase, H3 lysine-79 specific Q8TEK3
L Ubiquitin P62979
DNA:
Chain Variant Length
I 601 144
J 601 145

H3

H4

H2A

H2B

DNA

Other


Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.