6BUZ

Cryo-EM structure of CENP-A nucleosome in complex with kinetochore protein CENP-N

Exp. Method EM
Resolution, Å 3.92
Release Date 2017-12-20
Update Date 2024-03-13
Authors Chittori, S., Hong, J., Saunders, H., Feng, H., Ghirlando, R., Kelly, A.E., Bai, Y., Subramaniam, S.
Publication PMID: 29269420
doi 10.1126/science.aar2781
RCSB PDB 6BUZ
EMDB EMD-7293
Q-score 0.372
Core histones in Nucleosome-like particles:
Side Chain HistoneDB Variant Taxa UID
H3 Proximal A cenH3 Homo sapiens P49450
Distal E cenH3 Homo sapiens P49450
H4 Proximal B canonical H4 Homo sapiens P62805
Distal F canonical H4 Homo sapiens P62805
H2A Proximal C canonical H2A Homo sapiens P04908
Distal G canonical H2A Homo sapiens P04908
H2B Proximal D canonical H2B Homo sapiens P06899
Distal H canonical H2B Homo sapiens P06899
Other proteins:
Chain Name UID
N Maltose-binding periplasmic protein, Centromere protein N chimera P0AEX9
DNA:
Chain Variant Length
I 601 145
J 601 145

H3

H4

H2A

H2B

DNA

Other


Aligned nucleosome coordinates

Nucleosome reference frame definition

DNA relative twist and local base step parameters

Sequence on X-axis is numbered relative to the dyad base pair.

Atomic contacts between nucleosomal entities.


Hover the mouse cursor on the barplot to show contact information. Oncogenic mutations of non-histone proteins in contact are highlighted.